Testing catalase activity

 Essay in Testing catalase activity

Screening Catalase Activity

Prepared by Anel Behric

For professor Goodrow

General Biology Lab

Mohawk Valley Community College

10/14/2014 6: 00 PM


This experiment was conducted to measure the breakdown of base hydrogen peroxide (H2O2) by catalase, a specific enzyme that breaks down substrates of (H2O2). Molecules are for us and mother nature. They move about constantly which in turn causes them to be part of random accident, which develop chemical reactions. This is actually needed to maintain life. These collisions are influenced by many different physical factors, including temperature or concentration. In the event that more elements are concentrated in a place, the more unique collisions may happen. In the event that temperature is higher the reaction is hasten, thus leading to more accidents. Enzymes are catalyst mainly because they speed up reaction prices. They are shaped a specific method, so whenever they collide they fit perfectly into one another. Nutrients are protein that speed up chemical reactions by simply decreasing the power of account activation. Enzyme catalase convert hydrogen peroxide in bodies into oxygen and water. This is often visually noticed when hydrogen peroxide combined, and the peroxide bubbles. Catalyst is material that attracts molecules, this kind of increases likelihood of a collision. Materials employed for this research:

LoggerPropH buffers

Vernier O2 Sensorenzyme suspension system

10 milliliters graduated cylindertest tubes

Snow Packheating protect

250 cubic centimeters Nalgene bottlethermometer

3 test pontoons were put into a holding rack and labeled appropriately. Each check tube was filled with a few mL of 3% of H2O2 and 5 cubic centimeters of pure water. Each tube was filled utilizing a disposable pipette with 15 drops of enzymes postponement, interruption to Nalgene chamber for every observation. Tube one was poured in Nalgene step, and UNITED KINGDOM Gas Sensor was placed on top, and chamber was swirled to combine the enzymes. Same measures were performed with conduit two and three. Data was recorded in LoggerPro. Portion two screening consisted of Several concentrations of enzymes suspension system. To study the effects of enzyme concentration on rate of reaction, several test pontoons were every filled with your five mL of 3% hydrogen peroxide and 5 milliliters of drinking water. Five drops of enzyme suspension had been dropped in Nalgene jar, then T-MOBILE analyzer was placed on best. Mixture was swirled to get 5 minutes. Info was recorded same as in previous processes. Next observation covered ten drops of enzyme suspension and data was recorded. Observation 3 had 12-15 drops of enzyme answer and data was recorded. Twenty drops of enzyme answer were added in the fourth observation and data was written. In part three the effect of temperature for the reactions were tested. To check the effect of temperature on reaction level, three check tubes were each filled with 5 cubic centimeters of 3% hydrogen peroxide and 5 mL of water. Test out tube one particular was put into ice and ice drinking water to cool it into (Temperature of 0-5 C). Test tube two was measured for room temperature (20-25 C). Test pipe three was placed on a heating pad to increase temperature to (30-35 C).

Results intended for Part 1:

Test Tube Number

Slope of Linear Portion (m)


zero. 0026803

a couple of

0. 007677


0. 16337

Benefits for Component 2 (Concentration of Enzymes):

Drops of Enzymes Suspension

Initial Effect Rate (m)


zero. 18454


0. 16682


0. 16228


0. 16572

Results for part a few (Effect of Temperature on Reaction):

Heat Measured

Level of Initial reaction (m)


zero. 16252


0. 1658


zero. 030945

Digestive enzymes are responsible for most chemical reactions that take place. They are made up of healthy proteins and are regarded biocatalysts. (Jubenville. ) Biocatalysts can be described as once enzymes are used as factors to cause chemical reactions. (Novasep. ) Nutrients are referred to as catalyst as they are able to accelerate reaction costs without being destroyed or improved. They are able to encourage chemical reactions by...

Cited: http://www.novasep.com/misc/glossary.asp?defId=49. (Novasep. ) " Enzymes. " Digestive enzymes. Biocatalysts: Definition of Biocatalysts in Novasep Glossary. " Biocatalysis: Definition of Biocatalysts in Novasep Glossary.

http://www.tuberose.com/Enzymes.html>. (Enzymes. ) " Common questions


http://www.houston-enzymes.com/learn/faq.php. (Huston. ) Jubenville, Robert B., and Richard G. Thomas

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